A study was performed to assess the validity of the BAX automated polymerase chain reaction system (DuPont Nutrition & Health, Wilmington, Delaware) to detect the shedding of Salmonella species in bovine fecal samples. A total of 133 bovine fecal samples that were submitted to the Cornell University Animal Health Diagnostic Laboratory for Salmonella culture were also tested in the BAX system with a modified version of the manufacturer’s enrichment protocol. Using culture as the gold standard test, the BAX system was found to have a sensitivity of 85.7% and a specificity of 90.5%. There was excellent agreement ( = 0.71, standard error = 0.072) and no significant differences between the 2 methods (McNemar 2 = 0.180).
via Journal of Veterinary Diagnostic Investigation recent issues http://vdi.sagepub.com/cgi/content/abstract/25/6/756?rss=1
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